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primary antibodies against zonula occludens-1 (zo-1) and claudin-5  (Thermo Fisher)


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    Thermo Fisher primary antibodies against zonula occludens-1 (zo-1) and claudin-5
    Primary Antibodies Against Zonula Occludens 1 (Zo 1) And Claudin 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against zonula occludens-1 (zo-1) and claudin-5/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    primary antibodies against zonula occludens-1 (zo-1) and claudin-5 - by Bioz Stars, 2026-02
    90/100 stars

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    ( A ) Representative plot of oxygen consumption rate by a bezafibrate-treated and untreated 22qDS iBBB line during the Seahorse XFe96 Analyzer mitochondrial stress test ( n = 4 22qDS lines, 7 replicates per condition). ( B ) Quantification of OXPHOS parameters for each 22qDS line comparing bezafibrate-treated and untreated conditions ( n = 4 22qDS lines, 7 replicates per condition, paired t test). ( C ) Schematic is describing key regulators of mitochondrial dynamics. ( D ) Quantification of gene transcripts for fusion and fission ( n = 4 22qDS lines, paired t test). ( E ) Representative time course of TEER fold change following bezafibrate treatment in a 22qDS iBBB line, normalized to untreated ( n = 6-11 replicates per condition, two-way ANOVA). ( F ) Quantification of TEER at 36 hours following bezafibrate treatment ( n = 4 22qDS lines, 3-4 replicates per condition – 2 experiments per line, paired t test). ( G ) Immunofluorescence of <t>claudin-5</t> (green) in 22qDS in untreated (top) compared to bezafibrate treatment (bottom). Error bars ± SEM. Scale bar: 10 μm.
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    ( A ) Representative plot of oxygen consumption rate by a bezafibrate-treated and untreated 22qDS iBBB line during the Seahorse XFe96 Analyzer mitochondrial stress test ( n = 4 22qDS lines, 7 replicates per condition). ( B ) Quantification of OXPHOS parameters for each 22qDS line comparing bezafibrate-treated and untreated conditions ( n = 4 22qDS lines, 7 replicates per condition, paired t test). ( C ) Schematic is describing key regulators of mitochondrial dynamics. ( D ) Quantification of gene transcripts for fusion and fission ( n = 4 22qDS lines, paired t test). ( E ) Representative time course of TEER fold change following bezafibrate treatment in a 22qDS iBBB line, normalized to untreated ( n = 6-11 replicates per condition, two-way ANOVA). ( F ) Quantification of TEER at 36 hours following bezafibrate treatment ( n = 4 22qDS lines, 3-4 replicates per condition – 2 experiments per line, paired t test). ( G ) Immunofluorescence of <t>claudin-5</t> (green) in 22qDS in untreated (top) compared to bezafibrate treatment (bottom). Error bars ± SEM. Scale bar: 10 μm.
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    Image Search Results


    ( A ) Representative plot of oxygen consumption rate by a bezafibrate-treated and untreated 22qDS iBBB line during the Seahorse XFe96 Analyzer mitochondrial stress test ( n = 4 22qDS lines, 7 replicates per condition). ( B ) Quantification of OXPHOS parameters for each 22qDS line comparing bezafibrate-treated and untreated conditions ( n = 4 22qDS lines, 7 replicates per condition, paired t test). ( C ) Schematic is describing key regulators of mitochondrial dynamics. ( D ) Quantification of gene transcripts for fusion and fission ( n = 4 22qDS lines, paired t test). ( E ) Representative time course of TEER fold change following bezafibrate treatment in a 22qDS iBBB line, normalized to untreated ( n = 6-11 replicates per condition, two-way ANOVA). ( F ) Quantification of TEER at 36 hours following bezafibrate treatment ( n = 4 22qDS lines, 3-4 replicates per condition – 2 experiments per line, paired t test). ( G ) Immunofluorescence of claudin-5 (green) in 22qDS in untreated (top) compared to bezafibrate treatment (bottom). Error bars ± SEM. Scale bar: 10 μm.

    Journal: bioRxiv

    Article Title: Neurovascular mitochondrial susceptibility impacts blood-brain barrier function and behavior

    doi: 10.1101/2024.02.15.580544

    Figure Lengend Snippet: ( A ) Representative plot of oxygen consumption rate by a bezafibrate-treated and untreated 22qDS iBBB line during the Seahorse XFe96 Analyzer mitochondrial stress test ( n = 4 22qDS lines, 7 replicates per condition). ( B ) Quantification of OXPHOS parameters for each 22qDS line comparing bezafibrate-treated and untreated conditions ( n = 4 22qDS lines, 7 replicates per condition, paired t test). ( C ) Schematic is describing key regulators of mitochondrial dynamics. ( D ) Quantification of gene transcripts for fusion and fission ( n = 4 22qDS lines, paired t test). ( E ) Representative time course of TEER fold change following bezafibrate treatment in a 22qDS iBBB line, normalized to untreated ( n = 6-11 replicates per condition, two-way ANOVA). ( F ) Quantification of TEER at 36 hours following bezafibrate treatment ( n = 4 22qDS lines, 3-4 replicates per condition – 2 experiments per line, paired t test). ( G ) Immunofluorescence of claudin-5 (green) in 22qDS in untreated (top) compared to bezafibrate treatment (bottom). Error bars ± SEM. Scale bar: 10 μm.

    Article Snippet: Primary antibody rabbit polyclonal antibodies against claudin-5 (Life Technologies) was diluted 1:300 in 3% normal donkey serum and incubated overnight at 4°C.

    Techniques: Immunofluorescence

    ( A ) Quantification of mitochondrial content by Mitotracker fluorescence ( n = 7). ( B ) Representative EM images and ( C ) quantification of intramitochondrial density in BBB-ECs from treated and untreated 22qMc. ( D ) Representative immunofluorescent staining hippocampal vessels for leakiness (IgG, red) and claudin-5 (green). ( E ) Quantification of BBB leakiness as measured by serum IgG extravasation and ( F ) quantification of claudin-5 in hippocampal vessels in treated and untreated 22qMc ( n = 7-8). Error bars ± SEM. Scale bar: *, p < 0.05, ** p < 0.01 by nested t test.

    Journal: bioRxiv

    Article Title: Neurovascular mitochondrial susceptibility impacts blood-brain barrier function and behavior

    doi: 10.1101/2024.02.15.580544

    Figure Lengend Snippet: ( A ) Quantification of mitochondrial content by Mitotracker fluorescence ( n = 7). ( B ) Representative EM images and ( C ) quantification of intramitochondrial density in BBB-ECs from treated and untreated 22qMc. ( D ) Representative immunofluorescent staining hippocampal vessels for leakiness (IgG, red) and claudin-5 (green). ( E ) Quantification of BBB leakiness as measured by serum IgG extravasation and ( F ) quantification of claudin-5 in hippocampal vessels in treated and untreated 22qMc ( n = 7-8). Error bars ± SEM. Scale bar: *, p < 0.05, ** p < 0.01 by nested t test.

    Article Snippet: Primary antibody rabbit polyclonal antibodies against claudin-5 (Life Technologies) was diluted 1:300 in 3% normal donkey serum and incubated overnight at 4°C.

    Techniques: Fluorescence, Staining